Combinatorial metabolic pathway assembly approaches and toolkits for modular assembly

Synthetic Biology is a rapidly growing interdisciplinary field that primarily built upon foundational advances in molecular biology combined with engineering design principles such as modularity and interoperability. The considers living systems programmable at the genetic level has been defined by development of new platform technologies methodological advances. A key concept driving Design-Build-Test-Learn cycle which provides systematic framework for building biological systems. One major application area synthetic biosynthetic pathway requires modular assembly different regulatory elements enzymes. In this review we provide an overview DNA describe compare plethora vitro vivo methods combinatorial engineering. Considerations part enzyme balancing are also presented, briefly discuss alternatives to intracellular including microbial consortia cell-free biosynthesis. Finally, computational tools automation argue deeper understanding many variables design, regulation cellular metabolism will allow more predictive